The Cadenza (hexaploid) and Kronos (tetraploid) exome-sequenced TILLING lines are distributed by two seed banks. askWheat does not take orders: this page sorts your lines by distributor and formats them for each order form. Requests, material transfer agreements and payment go directly to the distributor.
1Your lines
Filled in from askWheat when you follow an order link. You can also paste line IDs here, e.g.
Cadenza0313, Kronos3545, Cadenza1166.
2Order from each distributor
Cadenza lines: JIC Germplasm Resources Unit (SeedStor), UK
- Open the SeedStor TILLING request page and paste the list into the request box.
- Choose an MTA: Research Use Only, or Freedom to Operate for commercial work. Sign it when asked.
- Ask for wild-type Cadenza as a control if you need one.
£15 per line plus £15 handling (research use); £200 per line for commercial use. Phytosanitary charges apply outside the UK/EU. Some lines may not have been regenerated; the GRU will suggest alternatives.
Kronos lines: Dubcovsky lab, UC Davis, USA
- Open the UC Davis TILLING order form and enter the line numbers (already formatted above).
- Download the MTA from the form, sign it and upload it as a PDF.
- Give a phone number for FedEx and, outside the US or Canada, your import requirements.
US$25 per line plus US$30 shipping. About 10–40 bulked M4 seeds per line.
3Before the seed arrives
- Import: wheat seed needs your country's import permit and usually a phytosanitary certificate; send the permit to the distributor with your order (Australia: DAFF import permit and post-entry quarantine).
- Genotype: lines are bulked M4 seed and may still segregate. Confirm the mutation in each plant (Ensembl Plants lists KASP primers for each EMS variant; askWheat gives the variant link).
- Background: each line carries thousands of other mutations (askWheat:
wheat_mutant_line). Backcross before phenotyping, and cross lines to combine A, B and D knockouts.
| Population | Ploidy | Distributor | ID format on the form |
|---|---|---|---|
| Cadenza | Hexaploid (A, B, D) | JIC SeedStor | Cadenza0313 |
| Kronos | Tetraploid (A, B) | UC Davis | 3545 |
Lines: Krasileva et al. (2017) PNAS doi:10.1073/pnas.1619268114. Prices and procedures as published on the distributors' pages in November 2026; check the order pages for changes. This page runs in your browser and sends nothing to askWheat.